Antiproliferative assay of human leucocyte interferon.
نویسندگان
چکیده
منابع مشابه
Studies on the Antiproliferative Action of Interferon
STUDIES ON THE ANTIPROLIFERATIVE ACTION OF INTERFERON. Effect on proteins synthesized in the Gl and S phase of the cell cycle in 2 anchorage-dependent cell lines. Dan Lundblad, Unit of Applied Cell and Molecular Biology, University of Umeå, S-901 87 Umeå. Interferons (IFNs) are a class of structurally related proteins first discovered to be produced by virus-infected cells. By now, several othe...
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Background:The premature termination of high producer clones, which will be killed due to cell proliferation and proteins production antagonism, is one of the basic drawback in recombinant proteins technology. Furtheremore, it is supposed some toxic proteins like interferon which we intended to clone and express, inhibit host cells’ proliferation. So, it is necessary to tightly control IFN-γ pr...
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At present there is a lack of standard criteria for the identification and evaluation of activity of antiviral compounds. Interferon was used to explore comparatively several laboratory methods. Interferon was produced in chick embryos and in chorioallantoic membranes suspended in vitro. Evaluation of interferon activity was performed by several methods: (i) percentage of inhibition of plaque-f...
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The purpose of these studies was to examine the antiproliferative properties of 16 recombinant human IFN-alpha B/D hybrids against various human tumor lines of different histological origin and to determine whether any of the hybrid molecules possessed immunomodulating activity that could active antitumor properties in peripheral blood monocytes of normal donors. Hybrids with the B domain at th...
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Four methods for the assay of human interferon in Vero cells were compared based on the inhibition of viral cytopathic effect (CPE) in tubes, the inhibition of CPE in microplates, the reduction of plaques, and the inhibition of quantitative hemadsorption. For inhibition of CPE, Sindbis virus, vesicular stomatitis virus, poliovirus type 2, and vaccinia virus were used for challenge. In the plaqu...
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ژورنال
عنوان ژورنال: The Tohoku Journal of Experimental Medicine
سال: 1987
ISSN: 0040-8727,1349-3329
DOI: 10.1620/tjem.151.309